Overview
Features:
- Advanced avidin/biotin technology. The Elite® ABC complex is smaller sized, very uniform, and highly active, allowing more ease of access for binding to some biotinylated target
- Greatest sensitivity, low background. The VECTASTAIN ® Elite® ABC system is easily the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is roughly 5 occasions more sensitive compared to original VECTASTAIN ® ABC Package with similar low background.
- Economical. Greater sensitivity results in less expensive per slide
- Available without ("Standard" package) or with biotinylated species-specific or universal secondary antibodies
- Obtainable in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Package reagents supply the same high sensitivity and occasional background because the traditional VECTASTAIN ® Elite® ABC Package reagents.
Package Contents:
- 2 ml Reagent A
- 2 ml Reagent B
- 1 ml biotinylated horse anti-mouse IgG
- 3 ml normal horse serum
Package contains sufficeint reagents to stain roughly 500-1000 sections.
More Information
Country of Manufacture
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
ABC Elite (Avidin/Biotin) Systems
Information
Details
Using more than 50,000 citations to the credit, the VECTASTAIN ® ABC package remains broadly popular. In line with the versatile avidin-biotin complex interaction, the machine is modular, and together with our choice of secondary antibodies, can hold several primary antibody and tissue species. Our ABC kits are economical and then be considered a staple product in almost any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These traits, with the high sensitivity and occasional reputation of the VECTASTAIN ® ABC systems, result in the peroxidase enzyme a frequent choice in lots of applications. (eg. In neural tissue, the peroxidase product is frequently preferred since it gives more consistent labeling of both cell physiques and procedures.)
VECTASTAIN ® Elite® ABC System
- Advanced avidin-biotin technology: The Elite® ABC complex is smaller sized, very uniform, and highly active, allowing more ease of access for binding to some biotinylated target
- Greatest sensitivity, low background: The VECTASTAIN ® Elite® ABC system is easily the most sensitive avidin-biotin complex-based peroxidase system. The Elite® ABC series is roughly 5 occasions more sensitive compared to original VECTASTAIN ® ABC Package with similar low background.
- Economical: Greater sensitivity results in less expensive per slide.
- Available without ( Standard package) or with biotinylated species specific or universal secondary antibodies.
- Obtainable in Ready-To-Use (R.T.U.) formats. Prediluted, stabilized working solutions of Elite® ABC Package reagents supply the same high sensitivity and occasional background because the traditional VECTASTAIN ® Elite® ABC Staining Package reagents.
VECTASTAIN ® Elite® ABC Package Components:
- 3 ml normal horse serum
- 1 ml of concentrated biotinylated horse anti-mouse IgG secondary antibody
- 2 ml all of concentrated A and B reagents.
Sufficient reagents to stain roughly 500-1000 sections.
The Avidin Biotin Complex Method
The VECTASTAIN ® ABC systems are very sensitive because of the form and quantity of active enzyme molecules connected using the preformed Avidin/Biotinylated enzyme Complex. This ABC complex uses two important qualities of avidin: 1) an extraordinarily high interest in biotin (more than one million occasions greater than antibody for many antigens), and a pair of) four biotin-binding sites. These qualities allow macromolecular complexes (ABCs) to become created by mixing Avidin DH (Reagent A) using its paired biotinylated enzyme (Reagent B) just before use. The ABC reagent, once created, remains stable for a lot of hrs after formation and can be used as a few days after preparation.
The VECTASTAIN ® ABC Reagent may be used to identify any molecule that's biotinylated. This property provides the avidin-biotin complex (ABC) method great versatility in the kinds of targets that may be detected along with the kinds of applications that can be used. Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands could be effectively visualized in applications for example:
- Tissue staining
- Multiple labeling (Multiplex IHC)
- Western blotting
- Southern and northern blotting
- In situ hybridization recognition (Ant)
- Enzyme immunoassays (ELISA)
- Neuronal tracing
All applications take advantage of the high sensitivity, low background, reproducibility, and economy from the VECTASTAIN ® ABC system.
Selecting a VECTASTAIN ® ABC Package
1) Choose an enzyme system:
The VECTASTAIN ® ABC Kits can be found with a range of three different enzyme systems
- Peroxidase (ABC, Elite® ABC, RTU or Kits)
- Alkaline Phosphatase (ABC-AP)
- Glucose Oxidase (ABC-GO)
2) Pick the biotinylated secondary antibody:
To identify a biotinylated target, you'll need just the ABC Reagent within the Standard Package, adopted by a suitable substrate.
To identify an unlabeled primary antibody, you may need a biotinylated secondary antibody that binds towards the primary antibody species selected, the ABC Reagent, as well as an appropriate substrate. For instance, to identify a principal antibody produced in rabbit, the right option is a VECTASTAIN ® ABC Package designated “Rabbit IgG”.
For further versatility and convenience, Vector Laboratories offers secondary antibody items that recognize several types of primary antibody. Our Universal Biotinylated Anti-Mouse/Rabbit IgG secondary antibody (Cat. No. BA-1400) is really a cocktail of biotinylated anti-mouse IgG and anti-rabbit IgG, created for use with rabbit and mouse primary antibodies. This antibody shouldn't be accustomed to stain tissues from rodents or rabbits because of its reactivity with IgG contained in the tissues of those species. The Universal Pan-Specific Anti- Mouse/Rabbit/Goat secondary antibody (Cat. No. BA-1300) (that is incorporated within our VECTASTAIN ® Universal Kits) is really a cocktail of biotinylated anti-mouse IgG, anti-rabbit IgG, and anti-goat IgG that recognizes mouse, rabbit, and goat primary antibodies. This secondary antibody shouldn't be accustomed to stain rat, mouse, or any other rodent, rabbit, goat, bovine, or sheep tissue because of potential reactivity with endogenous IgG.
3) Select a convenient format:
Finally, several kits can be found in prediluted, stabilized formats for convenience and simplicity of use. See kits designated R.T.U. or Ready-To-Use.
Consider Species Mix-Reactivity
When selecting the perfect recognition system for the application, you should consider not just the types of the main antibody but the types of the tissue under examination. When the types of the main antibody and also the types of the tissue are carefully related (e.g. rat and mouse), the biotinylated secondary antibody may mix interact with endogenous IgG within the tissue section resulting in background staining. The next three options minimize background staining in such cases:
- Biotinylate the main antibody (ProtOn™ Biotin Labeling Package, Cat. No. PLK-1202) and identify having a Standard VECTASTAIN ® Elite® ABC Package, PK-6100, (only includes the avidin-biotin complex, or ABC reagent).
- Make use of a biotinylated secondary antibody particularly adsorbed to get rid of mix-reacting antibodies of carefully-related species (e.g. biotinylated anti-mouse IgG, rat adsorbed, Cat. No. BA-2001).
- Make use of the M.O.M.™ Immunodetection System for applying mouse primary antibodies on mouse tissue.
Substrates
After selecting the VECTASTAIN ® ABC Package for the application, pick a substrate that suits the enzyme system from the package.
