Acetate buffer ph 4.5 recipe

I'm a bit confused in preparing acetate buffer using na acetate and acetic acidity. i have to prepare 10 mM na acetate buffer pH 4. and 25 mM Na Acetate buffer ph 4.5 and something with 25 mM Na Acetate buffer ph 4.5 that contains .4 M Nacl. just how much volume of sodium acetate and acetic acidity must i add to obtain the exact pH. can one prepare concentrated solutions and then dilute them as reported by the use.

kindly guide me about this

thanks in anticipation

QUOTE (professional @ May 15 2008, 01ᛂ PM)

I'm a bit confused in preparing acetate buffer using na acetate and acetic acidity. i have to prepare 10 mM na acetate buffer pH 4. and 25 mM Na Acetate buffer ph 4.5 and something with 25 mM Na Acetate buffer ph 4.5 that contains .4 M Nacl. just how much volume of sodium acetate and acetic acidity must i add to obtain the exact pH. can one prepare concentrated solutions and then dilute them as reported by the use.

kindly guide me about this

thanks in anticipation

Based on a recipe book from Roche Biochemicals

For any 3M solution pH 5.2

408.24g of Sodium Acetate (3H20) in 800ml of DD H20 and adjust pH 5.2 with glacial acetic acidity Allow it to be 1 liter sterilize by autoclaving

QUOTE (professional @ May 15 2008, 07ᛂ AM)

I'm a bit confused in preparing acetate buffer using na acetate and acetic acidity. i have to prepare 10 mM na acetate buffer pH 4. and 25 mM Na Acetate buffer ph 4.5 and something with 25 mM Na Acetate buffer ph 4.5 that contains .4 M Nacl. just how much volume of sodium acetate and acetic acidity must i add to obtain the exact pH. can one prepare concentrated solutions and then dilute them as reported by the use.

kindly guide me about this

thanks in anticipation

as you are preparing a few different concentrations of buffer and something with added salt, the best choice is always to make a greater concentration stock in the two pHs that you'll require.

to organize the stocks, i'd create a .1M acetate buffer each and every pH.

create a .1M stock of acetic acidity along with a .1M stock of na acetate.

mix them together until it reaches the prospective pH(s). by later on, regardless of what the ultimate volume or pH it it's still .1M buffer.

make one last dilutions in the stock (10x for 10mM and 4x for 25mM, and don't your investment salt).